Xylobone was freed from imprisonment when the Volcano on Donkey Kong Island erupted and by the order of its leader, Tiki Tong, he began stealing bananas from the island for their evil plot. After this occurred, Xylobone took control of the cliff area of the island and hypnotized its inhabitants. When the Kongs had defeated Xylobone's siblings Kalimba, the Maraca Gang, Gong-Oh, Banjo Bottom, and Wacky Pipes, they arrived at the cliff.
After adventuring through the cliff and avoiding its many obstacles, they reached Xylobone and met Thugly. In an effort to stop the two apes, the evil tiki hypnotized Thugly and ordered him to fight Donkey Kong and Diddy. After DK defeated the beast, Xylobone came out of Thugly and DK punched it, sending it to the sky.
The tiki later appears during the final boss fight with the eight other Tiki leaders where it transforms into one of Tiki Tong's hands and is used to fight the two apes. It is destroyed, however, during battle.