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Elder Princess Shroob is the older sister of Princess Shroob. She acts as a final boss (except "Shrowser") in Mario & Luigi: Partners in Time. After beating Princess Shroob, Peach is explaining to Mario and Luigi that they mustn't put the last cobalt star shard on the rest of it, as the Elder Princess Shroob was trapped inside by Peach. Then, baby Bowser appears, steals what he thinks is his treasure and puts the shards together. Elder Princess Shroob appears and turns him into a purple Shroob Mushroom. After she is defeated, it is revealed that baby tears cure everyone that were turned into Shroob Mushrooms.

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