||Based on his appearance in the SNES game Star Fox, he inhales, then blasts opponents with polygons.
|Barbara the Bat
||Jam with the Band
||Will jam on her guitar, thus generating shockwaves that attack the players.
||The main villain from Devil World, he points in different directions, causing the screen to shift that way, which moves the blast lines as well.
||Based on the game designer Will Wright and adviser in the SimCity series, he makes tall buildings pop up out of nowhere.
||In their pixelized forms, variously colored Excitebikers ride into opponents.
||From Metal Gear Solid, Gray Fox attacks with swift sword strikes, and also deflects projectiles.
||This popular Koopa Troopa from the Mario series will toss hammers at your opponents.
||From the Kururin series, this character acts as a temporary rotating platform.
|Infantry and Tanks
||A group of soldiers and tanks from the Nintendo Wars series attack but shooting down opponents; their appearances is based on Advance Wars sprites.
||The main character of the Golden Sun series, Isaac will use the synergy power "Move" to push players off the stage.
||Jeff pulls out his Multi Bottle Rocket 5 and launches rockets (4 small and 1 large) at his opponents.
||The main character of Drill Dozer, Jill drills into opponents.
|Kat & Ana
||A pair of sisters from the WarioWare series, Kat & Ana move across the screen, damaging opponents that touch them. They have the same purpose as Latias and Latios
||A popular enemy/helper from Kirby Super Star, he attacks with powerful jabs and punches.
|Lakitu and Spinies
||Also in their pixelized forms, Lakitu flies across the stage, throwing Spinies down at opponents.
||The main character of Mike Tyson's Punch-Out!!, Little Mac attacks with strong punches, and finishes off with his trademark uppercut.
||From Fire Emblem, the first Fire Emblem game to be released outside of Japan, Lyn appears taking a huge slash out of opponents.
||From the series of the same name, the Metroid latches onto opponents and sucks away their health.
||A mole from the Animal Crossing series who, just like in the games, gives long and boring lectures, which tend to block either the top half of the screen, or the player damage levels, and sometimes the dialogues interact with certain events in the battle. Like Nintendog, he isn't a positive effect for anyone.
||The Nintendog was the first Assist Trophy character revealed. However, it is one of the few characters that aren't positive for either the tosser or his opponents, as the dog will block the screen.
|Ray MK III
||Will go around the screen shooting the summoner's opposition.
||Sin & Punishment
||From Sin and Punishment: Successor of the Earth, Saki attacks using his Sword Cannon, but shooting opponents and slashing them.
||This famed F-Zero racer and Captain Falcon's rival will wield his sword and slash at your opponents.
|Shadow the Hedgehog
||Will slow down time.
||The Legendary Starfy
||From the Densetsu no Stafy series, Stafy attacks by spinning, but is one of the few Assist Trophies that can be attacked back.
||The Legend of Zelda
||Tingle has several different effects. He can:
- Inflate balloons, which can lift characters, but not damage them.
- Spawn many Hammers and Golden Hammers.
- Make the floor of the stage slippery, making everyone (including the summoner) slip.
- Be lit on fire, causing everyone to act as if they ate Superspicy Curry.
- Cause a flower effect, and make the camera zoom in on the summoner.
||From the Mario series, Waluigi stomps his opponents into the ground, then finishes off with either a powerful kick or by spinning his tennis racket.