Pauline

Pauline (known as Lady in Japan) is Mario's first love interest, she is from the original Donkey Kong. She is one of the very few major damsels in distress who is not a princess but rather a person of non-royalty. Pauline is Miyamoto's first female character, as well as Nintendo's first female.

Except for a few cameos no more was seen of her since the original Donkey Kong game, other than remakes of the same game.

After that game, Pauline was replaced by Peach, and the characters Mario, Donkey Kong separated into separate franchises. However in many Mario spin off games Donkey Kong either appears as a host or a playable character, but Pauline yet still hasn't been in a Mario spin off game.

After 13 years missing of Donkey Kong '94(latest remake), Pauline made a new appeareance on the game Mario vs. Donkey Kong 2: March of the Minis, for the Nintendo DS. In the game, she plays the major role of damsel in distress once again. However in this game it is displayed as Mario and Pauline being friends rather than a couple. Yet in the story they both seem to show affection to each other.

Pauline has also made cameo appearances in Tetris DS, and her first game was released for the Virtual Console two months after Mario vs. Donkey Kong 2: March of the Minis was released.